Obtaining and characterization of spheroids using colon adenocarcinoma SW480 cells

Sung H, Ferlay J, Siegel RL, Laversanne M, Soerjomataram I, Jemal A, et al. Global Cancer Statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin. 2021;71(3):209-49. https://doi.org/10.3322/caac.21660

Mazzoleni G, Di Lorenzo D, Steimberg N. Modelling tissues in 3D: the next future of pharmaco-toxicology and food research? Genes Nutr. 2009;4(1):13-22. https://doi.org/10.1007/s12263-008-0107-0

Imamura Y, Mukohara T, Shimono Y, Funakoshi Y, Chayahara N, Toyoda M, et al. Comparison of 2D- and 3D-culture models as drug-testing platforms in breast cancer. Oncol Rep. 2015;33(4):1837-43. https://doi.org/10.3892/or.2015.3767

Lovitt CJ, Shelper TB, Avery VM. Advanced cell culture techniques for cancer drug discovery. Biology. 2014;3(2):345-67. https://doi.org/10.3390/biology3020345

Breslin S, O’Driscoll L. Three-dimensional cell culture: the missing link in drug discovery. Drug Discov Today. 2013;18(5-6):240-9. https://doi.org/10.1016/j.drudis.2012.10.003

Kapałczyńska M, Kolenda T, Przybyla W, Zajaczkowska M, Teresiak A, Filas V, et al. 2D and 3D cell cultures – a comparison of different types of cancer cell cultures. Arch Med Sci. 2018;14(4):910-9. https://doi.org/10.5114/aoms.2016.63743

Ravi M, Paramesh V, Kaviya SR, Anuradha E, Paul Solomon FD. 3D cell culture systems: advantages and applications. J Cell Physiol. 2015;230(1):16-26. https://doi.org/10.1002/jcp.24683

Virgone-Carlotta A, Lemasson M, Mertani HC, Diaz J-J, Monnier S, Dehoux T, et al. In-depth phenotypic characterization of multicellular tumor spheroids: Effects of 5-fluorouracil. PLoS One. 2017;12(11):e0188100. https://doi.org/10.1371/journal.pone.0188100

Moreno-Londoño AP, Castañeda-Patlán MC, Sarabia-Sánchez MA, Macías-Silva M, Robles-Flores M. Canonical Wnt pathway is involved in chemoresistance and cell cycle arrest induction in colon cancer cell line spheroids. Int J Mol Sci. 2023;24(6):5252. https://doi.org/10.3390/ijms24065252

Tomita N, Jiang W, Hibshoosh H, Warburton D, Kahn SM, Weinstein IB. Isolation and characterization of a highly malignant variant of the SW480 human colon cancer cell line. Cancer Res. 1992;52(24):6840-7. PMID: 1458472

Pognan F, Beilmann M, Boonen HCM, Czich A, Dear G, Hewitt P, et al. The evolving role of investigative toxicology in the pharmaceutical industry. Nat Rev Drug Discov. 2023;22(4):317-35. https://doi.org/10.1038/s41573-022-00633-x

Baião A, Dias S, Soares AF, Pereira CL, Oliveira C, Sarmento B. Advances in the use of 3D colorectal cancer models for novel drug discovery. Expert Opin Drug Discov. 2022;17(6):569-80. https://doi.org/10.1080/17460441.2022.2056162

Hewitt RE, McMarlin A, Kleiner D, Wersto R, Martin P, Tsokos M, et al. Validation of a model of colon cancer progression. J Pathol. 2000;192(4):446-54. https://doi.org/10.1002/1096-9896(2000)9999:9999%3C::aid-path775%3E3.0.co;2-k

Maldonado ME, Bousserouel S, Gossé F, Minker C, Lobstein A, Raul F. Differential induction of apoptosis by apple procyanidins in TRAIL-sensitive human colon tumor cells and derived TRAIL-resistant metastatic cells. J Cancer Mol. 2009;5(1):21-30. Available from: https://bibliotecadigital.udea.edu.co/bitstream/10495/21982/3/MaldonadoMaria_2009_DifferentialInductionApoptosis.pdf

Ramirez V, Arango SS, Maldonado ME, Uribe D, Aguillón JA, Quintero JP, et al. Biological activity of Passiflora edulis f. flavicarpa ethanolic leaves extract on human colonic adenocarcinoma cells. J Appl Pharm Sci. 2019;9(02):64-71. Available from: http://hdl.handle.net/20.500.12622/3232

Cottet-Rousselle C, Ronot X, Leverve X, Mayol JF. Cytometric assessment of mitochondria using fluorescent probes. Cytometry A. 2011;79(6):405-25. https://doi.org/10.1002/cyto.a.21061

Crowley LC, Marfell BJ, Scott AP, Waterhouse NJ. Quantitation of apoptosis and necrosis by annexin V binding, propidium iodide uptake, and flow cytometry. Cold Spring Harb Protoc. 2016;2016(11):953-7. https://doi.org/10.1101/pdb.prot087288