Presence and Persistence of HPV 58 Molecular Variations ORF E6 and ORF E7 Among Women With Normal Cytology in the Bogotá, Colombia Cohort
Keywords:
Papilloma human virus, follow-up studies, genomic structural variantsAbstract
Objective: To analyze the presence and persistence of E6/E7 HPV58 variations in women with prevalent HPV58 infection, with normal cytology, who belong to the Bogotá, Colombia cohort.
Methods: Cervical cytobrush was used on 34 HPV58 women, with normal cytology, who are part of the cohort base line; respective follow was performed. The HPV58 E67/E7 region was broadened by using E6F1-E7R1 and E7P1-E7P2 indicators. Variation analysis was carried out with automatic direct sequencing. HPV58 sequence reference was used to compare the sequences that had been obtained.
Results: In 27/34 samples, E6/E7 variations of HPV58 were successfully detected. A total of five different variations were detected, two of which had never been reported before (A169/T307/A694/G744/A761 and T307/ A694/G744/A761/G763). Elimination analysis revealed that 75% of variations had been eliminated with in two years of follow up, and that all variation had been eliminated at the end of six years of followup.
Conclusions: Two new variations of universal phylogenetic and epidemiologic noteworthiness were reported
Author Biographies
Óscar Buitrago, Instituto Nacional de Cancerología
Grupo de Investigación en Biología del Cáncer, Instituto Nacional de Cancerología, Bogotá D. C., Colombia
Nicolás Morales, Instituto Nacional de Cancerología
Grupo de Investigación en Biología del Cáncer, Instituto Nacional de Cancerología, Bogotá D. C., Colombia
Carolina Martín, Instituto Nacional de Cancerología
Grupo de Investigación en Biología del Cáncer, Instituto Nacional de Cancerología, Bogotá D. C., Colombia
Antonio Huertas, Instituto Nacional de Cancerología
Grupo de Investigación en Biología del Cáncer, Instituto Nacional de Cancerología, Bogotá D. C., Colombia
Pablo Moreno, Instituto Nacional de Cancerología
Grupo de Investigación en Biología del Cáncer, Instituto Nacional de Cancerología, Bogotá D. C., Colombia
Teresa Martínez, Instituto Nacional de Cancerología
Grupo de Investigación Epidemiológica, Instituto Nacional de Cancerología, Bogotá D. C., Colombia
Mónica Molano, Instituto Nacional de Cancerología
Grupo de Investigación en Biología del Cáncer, Instituto Nacional de Cancerología, Bogotá D. C., Colombia
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