Analysis of NK Cells in Peripheral Blood and Tumor Infiltrating Lymphocytes in Cervical Cancer Patients
Keywords:
Lymphocytes, tumor-infiltrating, killer cells, natural, uterine cervical neoplasmsAbstract
Objective: To understand the biologic and clinical importance of intratumoral natural killer cells CD16+CD56+CD3 and NKT CD16+CD56+CD3 cells in immune surveillance against cervical cancer.
Methods: To understand the significance of NK (CD16+CD56+CD3-) and NKT (CD16+CD56+CD3-) in immune surveillance against cervical cancer, we analysed 39 peripheral blood and 30 biopsy samples from cervical cancer patients, and 40 peripheral blood and 5 biopsy samples from healthy women with normal cytology. The frequencies of NK and NKT and HLA-I expression in keratinocytes were analysed by flow cytometry.
Results: In peripheral blood, a higher frequency of NK was observed in the patient group compared with the controls (p=0.002). However, this increase was not reflected in TILs (p=0,095). A significant reduction of HLA-I expression was observed in the patient group compared to the control group (p=0.019). A low number of NK infiltrated was observed in tumors of patients with HLA-I down regulation, but it was not significant (p=0.374). A low number of NK infiltration was associated with shorter survival, but it was not significant (p=0.275).
Conclusions: Our results show that although in peripheral blood an increase in NK population was observed in patient group, this increase was not reflected in TILs. It is possible that this inefficient migration of NK ́s into the tumor milieu could be related to the expression of immunosuppressive cytokines, in particular IL-10
Author Biographies
María A. Céspedes, Instituto Nacional de Cancerología
Grupo de Investigación en Biología del Cáncer, Instituto Nacional de Cancerología, Bogotá D. C., Colombia
Josefa A. Rodríguez, Instituto Nacional de Cancerología
Grupo de Investigación en Biología del Cáncer, Instituto Nacional de Cancerología, Bogotá D. C., Colombia
Mónica Medina, Instituto Nacional de Cancerología
Clínica de Ginecología, Instituto Nacional de Cancerología, Bogotá D. C., Colombia
María Bravo, Instituto Nacional de Cancerología
Grupo de Investigación en Biología del Cáncer, Instituto Nacional de Cancerología, Bogotá D. C., Colombia
Alba L. Cómbita, Instituto Nacional de Cancerología
Grupo de Investigación en Biología del Cáncer, Instituto Nacional de Cancerología, Bogotá D. C., Colombia
Departamento de Microbiología, Facultad de Medicina, Universidad Nacional de Colombia, Bogotá D. C., Colombia
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