Pre-cancerous gastric lesions in Colombian patients: association with IL-1B-511, IL-1RN, IL-10-919, IL-10-1082, TNF-a-308 genes polymorphisms, and anti-Helicobacter pylori cagA IgG antibodies
Keywords:
Interleukin-1beta, Interleukin 1 receptor antagonist, Interleukin-10, Tumor necrosis factor-alpha, Helicobacter pylori, Pre-cancerous gastric lesionAbstract
Objective: To evaluate the relationship of some of the most studied cytokines (IL-1B-511, IL-1RN intron-2-VNTR, TNF-α-308, IL-10-819, and IL-10-1082) with gastric cancer, as well as the presence of anti-Helicobacter pylori cagA IgG antibodies with pre-cancerous lesions in Colombian patients.
Materials and methods: A study was conducted on 185 patients with pre-cancerous lesions (atrophic gastritis, intestinal metaplasia and dysplasia), and 154 controls (non-atrophic gastritis), seen in hospitals in a high risk area, and another in a low risk area, for gastric cancer. Gastric biopsy specimens and blood samples were obtained. The genotyping of the polymorphisms was performed by allelic discrimination using real-time PCR, conventional PCR, and agarose electrophoresis (VNTR of IL-1RN intron 2). The serology of Helicobacter pylori and Helicobacter pylori cagA was determined by ELISA. A multinomial logistic regression was used in the statistical analysis.
Results: The IL-1B-511TT genotype was associated with intestinal metaplasia (OR=4.05; 95% CI; 1.35-12.10). No other relationships were observed between the different polymorphisms and pre-cancerous lesions. Infection due to a positive Helicobacter pylori cagA was associated with atrophic gastritis, intestinal metaplasia and dysplasia (OR=2.66; 13.70; 40.29, respectively).
Conclusion: The results suggest that, among the pro-inflammatory genotypes, the IL-1B-511TT would be associated with intestinal metaplasia, and that a positive Helicobacter pylori cagA serology could be a useful biomarker for the intervention and prevention of pre-cancerous lesions. Further studies are required in the Colombian population in order to evaluate the relationship found between IL1B-511 and intestinal metaplasia.
Author Biographies
Teresa Martínez, Instituto Nacional de Cancerología ESE
Grupo de Investigación Epidemiológica, Instituto Nacional de Cancerología ESE., Bogotá, D.C., Colombia
Gustavo A. Hernández, Instituto Nacional de Cancerología ESE
Grupo de Investigación Epidemiológica, Instituto Nacional de Cancerología ESE., Bogotá, D.C., Colombia
María Mercedes Bravo, Instituto Nacional de Cancerología ESE
Grupo de Biología del Cáncer, Instituto Nacional de Cancerología ESE., Bogotá, D.C., Colombia
Esperanza Trujillo, Instituto Nacional de Cancerología ESE
Grupo de Biología del Cáncer, Instituto Nacional de Cancerología ESE., Bogotá, D.C., Colombia
Jesús Pérez García, Universidad Libre
Facultad de Medicina, Universidad Libre, Laboratorio de Patología Clínica General del Norte, Barranquilla, Colombia
Juan C. Robayo, Fundación Santa Fe de Bogotá
Departamento de Gastroenterología, Fundación Santa Fe de Bogotá, Bogotá, D.C., Colombia
Margarita Camorlinga, Instituto Mexicano de Seguros Sociales
Laboratorio de Bacteriología, Unidad de Investigación Médica en Enfermedades Infecciosas y Parasitarias, Hospital de Pediatría CMN siglo XXI, Instituto Mexicano de Seguros Sociales, Ciudad de México, México
References
IARC. IARC monograph on the evaluation of carcinogenic risk to humans: schistosomes, liver flukes and Helicobacter pylori. Lión, Francia: IARC; 1994.
PeekR,BlaserM.Helicobacterpyloriandgastrointestinaltract adenocarcinomas. Nat Rev Cancer. 2002;2:28-37.
https://doi.org/10.1038/nrc703
Correa P. Human gastric carcinogenesis: a multistep and multifactorial process-First American Cancer Society Award Lecture on Cancer Epidemiology and Prevention. Cancer Res. 1992;52:6735-40.
Plummer M, van Doorn LJ, Franceschi S, Kleter B, Canzian F, Vivas J, et al. Helicobacter pylori cytotoxin-associated genotype and gastric precancerous lesions. J Natl Cancer Inst. 2007;99:1328-34.
https://doi.org/10.1093/jnci/djm120
Huang JQ, Zheng GF, Sumanac K, Irvine EJ, Hunt RH. Meta-analysis of the relationship between cagA seropositivity and gastric cancer. Gastroenterology. 2003;125: 1636-44.
https://doi.org/10.1053/j.gastro.2003.08.033
Ernst P. Review article: the role of inflammation in the pathogenesis of gastric cancer. Aliment Pharmacol Ther. 1999; 13 Suppl 1:13-8.
https://doi.org/10.1046/j.1365-2036.1999.00003.x
AmievaMR,El-OmarEM.Host-bacterialinteractionsin Helicobacter pylori infection. Gastroenterology. 2008;134:306-23.
https://doi.org/10.1053/j.gastro.2007.11.009
Peek RM, Jr., Fiske C, Wilson KT. Role of innate immunity in Helicobacter pylori-induced gastric malignancy. Physiol Rev. 2010;90:831-58.
Chang YW, Jang JY, Kim NH, Lee JW, Lee HJ, Jung WW, et al. Interleukin-1B (IL-1B) polymorphisms and gastric mucosal levels of IL-1beta cytokine in Korean patients with gastric cancer. Int J Cancer. 2005;114:465-71.
https://doi.org/10.1002/ijc.20724
El-Omar EM, Rabkin CS, Gammon MD, Vaughan TL, Risch HA, Schoenberg JB, et al. Increased risk of noncardia gastric cancer associated with proinflammatory cytokine gene polymorphisms. Gastroenterology. 2003;124:1193-201.
https://doi.org/10.1016/S0016-5085(03)00157-4
Bonequi P, Meneses-Gonzalez F, Correa P, Rabkin CS , Camargo MC. Risk factors for gastric cancer in Latin America: a meta-analysis. Cancer Causes Control. 2013;24:217-31.
https://doi.org/10.1007/s10552-012-0110-z
Camargo MC, Mera R, Correa P, Peek RM, Jr., Fontham ET, Goodman KJ, et al. Interleukin-1beta and interleukin-1 receptor antagonist gene polymorphisms and gastric cancer: a meta-analysis. Cancer Epidemiol Biomarkers Prev. 2006;15:1674-87.
https://doi.org/10.1158/1055-9965.EPI-06-0189
Loh M, Koh KX, Yeo BH, Song CM, Chia KS, Zhu F, et al. Meta-analysis of genetic polymorphisms and gastric cancer risk: variability in associations according to race. Eur J Cancer. 2009;45:2562-8.
https://doi.org/10.1016/j.ejca.2009.03.017
Peleteiro B, Lunet N, Carrilho C, Duraes C, Machado JC, La Vecchia C, et al. Association between cytokine gene polymorphisms and gastric precancerous lesions: systematic review and meta-analysis. Cancer Epidemiol Biomarkers Prev. 2010;19:762-76.
https://doi.org/10.1158/1055-9965.EPI-09-0917
Persson C, Canedo P, Machado JC, El-Omar EM, Forman D. Polymorphisms in inflammatory response genes and their association with gastric cancer: A HuGE systematic review and meta-analyses. Am J Epidemiol. 2011;173:259-70.
https://doi.org/10.1093/aje/kwq370
Gorouhi F, Islami F, Bahrami H, Kamangar F. Tumour-necrosis factor-A polymorphisms and gastric cancer risk: a meta-analysis. Br J Cancer. 2008;98:1443-51.
https://doi.org/10.1038/sj.bjc.6604277
Zhang J, Dou C, Song Y, Ji C, Gu S, Xie Y, et al. Polymorphisms of tumor necrosis factor-alpha are associated with increased susceptibility to gastric cancer: a meta-analysis. J Hum Genet. 2008;53:479-89.
https://doi.org/10.1007/s10038-008-0273-3
Chen KF, Li B, Wei YG, Peng CJ. Interleukin-10 -819 promoter polymorphism associated with gastric cancer among Asians. J Int Med Res. 2010;38:1-8.
https://doi.org/10.1177/147323001003800101
Won HH, Kim JW, Kim MJ, Kim S, Park JH, Lee KA. Interleukin 10 polymorphisms differentially influence the risk of gastric cancer in East Asians and Caucasians. Cytokine. 2010;51:73-7.
https://doi.org/10.1016/j.cyto.2010.03.007
Torres J, Correa P, Ferreccio C, Hernandez-Suarez G, Herrero R, Cavazza-Porro M, et al. Gastric cancer incidence and mortality is associated with altitude in the mountainous regions of Pacific Latin America. Cancer Causes Control. 2013; 24:249-56.
https://doi.org/10.1007/s10552-012-0114-8
Pineros M, Pardo C, Gamboa O, Hernández GA. Atlas de mortalidad por cáncer en Colombia. Bogotá: Instituto Nacional de Cancerologia; Instituto Agustin Codazzi; República de Colombia Ministerio de Protección Social; 2010.
Rodas C, Gelvez N, Keyeux G. Mitochondrial DNA studies show asymmetrical Amerindian admixture in Afro-Colombian and Mestizo populations. Hum Biol. 2003;75:13-30.
https://doi.org/10.1353/hub.2003.0026
Bravo LE, van Doom LJ, Realpe JL, Correa P. Virulence associated genotypes of Helicobacter pylori: do they explain the African enigma? Am J Gastroenterol. 2002;97:2839-42.
https://doi.org/10.1111/j.1572-0241.2002.07031.x
De ST, Piazuelo MB, Shaffer CL, Schneider BG, Asim M, Chaturvedi R, et al. Phylogeographic origin of Helicobacter pylori is a determinant of gastric cancer risk. Gut. 2011;60: 1189-95.
https://doi.org/10.1136/gut.2010.234468
Dixon MF, Genta RM, Yardley JH, Correa P. Classification and grading of gastritis. The updated Sydney System. International Workshop on the Histopathology of Gastritis, Houston 1994. Am J Surg Pathol. 1996;20:1161-81.
https://doi.org/10.1097/00000478-199610000-00001
Filipe MI, Munoz N, Matko I, Kato I, Pompe-Kirn V, Jutersek A, et al. Intestinal metaplasia types and the risk of gastric cancer: a cohort study in Slovenia. Int J Cancer. 1994;57: 324-9.
https://doi.org/10.1002/ijc.2910570306
Rugge M, Correa P, Dixon MF, Hattori T, Leandro G, Lewin K, et al. Gastric dysplasia: the Padova international classification. Am J Surg Pathol. 2000;24:167-76.
https://doi.org/10.1097/00000478-200002000-00001
Johnson VJ, Yucesoy B, Luster MI. Genotyping of single nucleotide polymorphisms in cytokine genes using real-time PCR allelic discrimination technology. Cytokine. 2004;27: 135-41.
https://doi.org/10.1016/j.cyto.2004.05.002
Martinez T, Hernández G, Bravo M, Trujillo E, Quiroga A, Robayo J, et al. Polimorfismos genéticos de interleucinas 1L-IB-511, IL-1RN, IL-10, factor de necrosis tumoral a-308 e infección por Helicobacter pylori CagA positivo en cáncer gástrico y úlcera duodenal en diferentes poblaciones en Colombia. Revista Colombiana de Cancerología. 2011;15: 31-43.
Perri F, Piepoli A, Bonvicini C, Gentile A, Quitadamo M, Di Candia M, et al. Cytokine gene polymorphisms in gastric cancer patients from two Italian areas at high and low cancer prevalence. Cytokine. 2005;30:293-302.
https://doi.org/10.1016/j.cyto.2005.01.011
Tarlow JK, Blakemore AI, Lennard A, Solari R, Hughes HN, Steinkasserer A, et al. Polymorphism in human IL-1 receptor antagonist gene intron 2 is caused by variable numbers of an 86-bp tandem repeat. Hum Genet. 1993;91:403-4.
https://doi.org/10.1007/BF00217368
Camorlinga-Ponce M, Torres J, Perez-Perez G, Leal-Herrera Y, Gonzalez-Ortiz B, Madrazo de la Garza A, et al. Validation of a serologic test for the diagnosis of Helicobacter pylori infection and the immune response to urease and CagA in children. Am J Gastroenterol. 1998;93:1264-70.
https://doi.org/10.1111/j.1572-0241.1998.00407.x
Camorlinga-Ponce M, Flores-Luna L, Lazcano-Ponce E, Herrero R, Bernal-Sahagun F, Abdo-Francis JM, et al. Age and severity of mucosal lesions influence the performance of serologic markers in Helicobacter pylori-associated gastroduodenal pathologies. Cancer Epidemiol Biomarkers Prev. 2008;17:2498-504.
https://doi.org/10.1158/1055-9965.EPI-08-0289
Martinez T, Hernández G, Bravo M, Trujillo E, Quiroga A, Albis R, et al. Asociación de los polimorfismos IL-1B-511 e IL-1RN, y Helicobacter pylori cagA positivo con cáncer gástrico en una zona de riesgo alto en Colombia. Revista Médica de Chile. 211;139:1313-21.
https://doi.org/10.4067/S0034-98872011001000010
Kupcinskas L, Wex T, Kupcinskas J, Leja M, Ivanauskas A, Jonaitis LV, et al. Interleukin-1B and interleukin-1 receptor antagonist gene polymorphisms are not associated with premalignant gastric conditions: a combined haplotype analysis. Eur J Gastroenterol Hepatol. 2010;22:1189-95.
https://doi.org/10.1097/MEG.0b013e32833cf3d5
El-Omar EM. The importance of interleukin 1beta in Helicobacter pylori associated disease. Gut. 2001;48:743-7.
https://doi.org/10.1136/gut.48.6.743
Rad R, Dossumbekova A, Neu B, Lang R, Bauer S, Saur D, et al. Cytokine gene polymorphisms influence mucosal cytokine expression, gastric inflammation, and host specific colonisation during Helicobacter pylori infection. Gut. 2004; 53:1082-9.
https://doi.org/10.1136/gut.2003.029736
Figueiredo C, Machado JC, Pharoah P, Seruca R, Sousa S, Carvalho R, et al. Helicobacter pylori and interleukin 1 genotyping: an opportunity to identify high-risk individuals for gastric carcinoma. J Natl Cancer Inst. 2002;94:1680-7.
https://doi.org/10.1093/jnci/94.22.1680
Garza-Gonzalez E, Bosques-Padilla FJ, El-Omar E, Hold G, Tijerina-Menchaca R, Maldonado-Garza HJ, et al. Role of the polymorphic IL-1B, IL-1RN and TNF-A genes in distal gastric cancer in Mexico. Int J Cancer. 2005;114:237-41.
https://doi.org/10.1002/ijc.20718
Machado JC, Figueiredo C, Canedo P, Pharoah P, Carvalho R, Nabais S, et al. A proinflammatory genetic profile increases the risk for chronic atrophic gastritis and gastric carcinoma. Gastroenterology. 2003;125:364-71.
https://doi.org/10.1016/S0016-5085(03)00899-0
Rad R, Prinz C, Neu B, Neuhofer M, Zeitner M, Voland P, et al. Synergistic effect of Helicobacter pylori virulence factors and interleukin-1 polymorphisms for the development of severe histological changes in the gastric mucosa. J Infect Dis. 2003; 188:272-81.
https://doi.org/10.1086/376458
Kamangar F, Cheng C, Abnet CC, Rabkin CS. Interleukin-1B polymorphisms and gastric cancer risk-a meta-analysis. Cancer Epidemiol Biomarkers Prev. 2006;15:1920-8.
https://doi.org/10.1158/1055-9965.EPI-06-0267
Torres MM, Acosta CP, Sicard DM, Groot de Restrepo H. Genetic susceptibility and risk of gastric cancer in a human population of Cauca, Colombia. Biomedica. 2004;24:153-62.
https://doi.org/10.7705/biomedica.v24i2.1261
Kato I, van Doorn LJ, Canzian F, Plummer M, Franceschi S, Vivas J, et al. Host-bacterial interaction in the development of gastric precancerous lesions in a high risk population for gastric cancer in Venezuela. Int J Cancer. 2006;119: 1666-71.
https://doi.org/10.1002/ijc.21979
Con SA, Con-Wong R, Con-Chin GR, Con-Chin VG, Takeuchi H, Valerin AL, et al. Serum pepsinogen levels, Helicobacter pylori CagA Status, and cytokine gene polymorphisms associated with gastric premalignant lesions in Costa Rica. Cancer Epidemiol Biomarkers. Prev 2007;16:2631-6.
https://doi.org/10.1158/1055-9965.EPI-07-0215
Leung WK, Chan MC, To KF, Man EP, Ng EK, Chu ES, et al. H. pylori genotypes and cytokine gene polymorphisms influence the development of gastric intestinal metaplasia in a Chinese population. Am J Gastroenterol. 2006;101:714-20.
https://doi.org/10.1111/j.1572-0241.2006.00560.x
Murphy G, Thornton J, McManus R, Swan N, Ryan B, Hughes DJ, et al. Association of gastric disease with polymorphisms in the inflammatory-related genes IL-1B, IL-1RN, IL-10, TNF and TLR4. Eur J Gastroenterol Hepatol. 2009;21:630-5.
https://doi.org/10.1097/MEG.0b013e3283140eea
Kato I, Canzian F, Franceschi S, Plummer M, van Doorn LJ, Lu Y, et al. Genetic polymorphisms in anti-inflammatory cytokine signaling and the prevalence of gastric precancerous lesions in Venezuela. Cancer Causes Control. 2006;17:1183-91.
https://doi.org/10.1007/s10552-006-0060-4
Zhu F, Loh M, Hill J, Lee S, Koh KX, Lai KW, et al. Genetic factors associated with intestinal metaplasia in a high risk Singapore-Chinese population: a cohort study. BMC Gastroenterol. 2009;9:1-9.
https://doi.org/10.1186/1471-230X-9-76
Gao L, Weck MN, Nieters A, Brenner H. Association between a pro-inflammatory genetic profile and the risk of chronic atrophic gastritis among older adults from Germany. Eur J Cancer. 2009;45:428-34.
https://doi.org/10.1016/j.ejca.2008.09.019
Pan F, Tian J, Pan YY, Zhang Y. Association of IL-10-1082 promoter polymorphism with susceptibility to gastric cancer: evidence from 22 case-control studies. Mol Biol Rep. 2012;39: 7143-54.
https://doi.org/10.1007/s11033-012-1546-7
Hatakeyama M. Oncogenic mechanisms of the Helicobacter pylori CagA protein. Nat Rev Cancer. 2004;4:688-94.
https://doi.org/10.1038/nrc1433
Groves FD, Perez-Perez G, Zhang L, You WC, Lipsitz SR, Gail MH, et al. Serum antibodies to Helicobacter pylori and the CagA antigen do not explain differences in the prevalence of precancerous gastric lesions in two Chinese populations with contrasting gastric cancer rates. Cancer Epidemiol Biomarkers Prev. 2002;11:1091-4.
Flores-Luna L, Camorlinga-Ponce M, Hernandez-Suarez G, Kasamatsu E, Martinez ME, Murillo R, et al. The utility of serologic tests as biomarkers for Helicobacter pylori-associated precancerous lesions and gastric cancer varies between Latin American countries. Cancer Causes Control. 2013;24:241-8.
How to Cite
Downloads
Downloads
Published
Issue
Section
License
Todos los derechos reservados.
Article metrics | |
---|---|
Abstract views | |
Galley vies | |
PDF Views | |
HTML views | |
Other views |