DNA damage as a potential marker in the clinical follow-up of female patients with treated cancer
DOI:
https://doi.org/10.35509/01239015.122Keywords:
Cancer, alkaline comet assay, neutral comet assay, DNA damageAbstract
Objective: To identify individual differences in the basal damage (DB) of peripheral leukocyte DNA from women with cancer in remission. Methods: Cross-sectional analytical study in which 24 women with cancer in remission from different locations and 24 supposedly healthy women participated. The alkaline comet assay and the neutral variant were used to
determine single-stranded breaks (DB-A), and double-stranded DNA breaks (DB-N), respectively. Results: Although there
were no differences between the mean values of DNA damage in patients and controls (DB-N: p = 0.43 and DB-A: p = 0.13),
41.6% of the patients presented an increase of one type or another of DNA breaks, with respect to the corresponding
cut-off points of the control women. DB-N was correlated with increasing age (r2 = 0.1833; r = 0.4281; p = 0.036) in the patients. DB-A was elevated in those who had received anticancer combination therapy (p = 0.024) and in those who were undergoing treatment with tamoxifen (p = 0.033); while it was decreased in those that consumed antioxidants (p = 0.006) and in those that combined tamoxifen and antioxidants (p = 0.020). Conclusions: Individual differences were identified in both types of DNA strand breaks that are of medical interest in the studied patients. Baseline DNA damage determined by comet assay is a potential tool in the clinical follow-up of cancer patients in remission.
Author Biography
Judith Beatriz Pupo Balboa, Laboratorio de Estrés Oxidativo. Centro Nacional de Genética Médica. Dirección: Ave. 31 Esq.146 No 3102, Reparto Cubanacán, CP 11400, La Habana. Cuba.
Ph.D..National Center of Medical Genetic, Oxidaive stress
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